TSHR Antibody (beta chain)

• Description:

  Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P16473

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human TSH Receptor beta chain protein was used as the immunogen for this TSHR antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  TSHRB/1405

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the TSHR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This TSHR antibody is available for research use only.

• Storage Conditions:

  Store the TSHR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSHR antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell surface

• Image Legend:

  IHC testing of FFPE human thyroid carcinoma with TSHR antibody (clone TSHRB/1405) . Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.