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Villin Antibody / VIL1

CAT: 0800-V3369-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3369-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Villin (VIL1) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA) ; binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF) -induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL for 60 min at RT, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
UniProt
P09327
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VIL1/1325
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Villin antibody is available for research use only.
Storage Conditions
Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic and cell surface
Image Legend
IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

UniProtKB · P09327

Villin-1

VILI_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09327
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VIL1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R9A7, Q53S11, Q96AC8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

Actin cappingActin-binding

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Ligand

Calcium

Cellular component

Cell projectionCytoplasmCytoskeleton

PTM

Phosphoprotein

Domain

Repeat
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