Villin Antibody

CAT: 0800-V8157-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V8157-20UGSize:20 µg
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Description
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P09327
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 179-311) was used as the immunogen for this Villin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VIL1/2376
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Villin antibody is available for research use only.
Storage Conditions
Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Villin antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
IHC staining of FFPE human colon carcinoma with Villin antibody (clone VIL1/2376) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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