VIL1 Antibody / Villin 1

• Description:

  Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.

• Specifications:

  Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells)

• UniProt:

  P09327

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 180-207 from the human protein were used as the immunogen for the VIL1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC-P, FACS

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the VIL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This VIL1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the VIL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the VIL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic and cell surface

• Image Legend:

  Western blot testing of 1) human HT-29, 2) human HepG2, 3) human COLO205 and 4) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.