CD20 Antibody

• Description:

  CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

• Specifications:

  Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  P11836

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human CD20 protein was used as the immunogen for this CD20 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This CD20 antibody is available for research use only.

• Storage Conditions:

  CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titering of the CD20 antibody may be required for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Predominantly cell surface with some cytoplasmic

• Image Legend:

  IHC testing of CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH6 citrate buffer for 20 min.