Lambda Antibody

CAT: 0800-V3152-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V3152-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format)
UniProt
P01701, P01842
Host
Mouse
Reactivity
Human
Immunogen
Purified human lambda light chain was used as the immunogen for this Lambda antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LAM03
Applications
ELISA
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Lambda antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Lambda antibody is available for research use only.
Storage Conditions
Store the Lambda antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the Lambda antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Location
Cell surface, cytoplasmic and secreted
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free Lambda Antibody (clone LAM03) . Confirmation of Integrity and Purity of the Antibody.

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