Lambda Light Chain Antibody

• Description:

  Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both together. Specific rearrangement of lambda light chain of immunoglobulins can lead to loss of some protein coding genes, which does not seem to be functionally relevant (while functionally relevant miR-650 can be overexpressed) . Using immunohistochemistry, it is possible to determine the relative abundance of B-cells expressing kappa and lambda light chains. If the lymph node or similar tissue is reactive, or otherwise benign, it should possess a mixture of kappa positive and lambda positive cells. If, however, one type of light chain is significantly more common than the other, the cells are likely all derived from a small clonal population, which may indicate a malignant condition, such as B-cell lymphoma. [Wiki]

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  P01701, P01842

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  N10/2

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Lambda Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Lambda Light Chain antibody is available for research use only.

• Storage Conditions:

  Store the Lambda Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The optimal dilution of the Lambda Light Chain antibody for each application should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell Surface, Cytoplasmic and Secreted

• Image Legend:

  IHC testing of FFPE human tonsil stained with Lambda Light Chain antibody (clone N10/2) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.