CD44 Antibody

• Description :

  Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V) . Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

• Specifications :

  Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P16070

• Host :

  Mouse

• Reactivity :

  Human, Mouse

• Immunogen :

  Purified CD44 antigen (PGp-1) from lymphocyte membrane was used as the immunogen for the CD44 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  DF1485

• Applications :

  IF, IHC-P, WB

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD44 antibody is available for research use only.

• Storage Conditions :

  Store the CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with CD44 antibody (clone DF1485) .