CD44 Antibody

• Description :

  CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Variations in CD44 are reported as cell surface markers for some breast and prostate cancer stem cells. In breast cancer research CD44+/CD24- expression is commonly used as a marker for breast CSCs and is used to sort breast cancer cells into a population enriched in cells with stem-like characteristics and has been seen as an indicator of increased survival time in epithelial ovarian cancer patients. [Wiki]

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• UniProt :

  P16070

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Stimulated human leukocytes were used as the immunogen for the CD44 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  SPM521

• Applications :

  WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD44 antibody is available for research use only.

• Storage Conditions :

  Store the CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  Western blot testing of human HeLa cell lysate with CD44 antibody (clone SPM521) . Expected molecular weight ~81 kDa.