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Cyclin B1 Antibody

CAT: 0800-V2946IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2946IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P14635
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CCNB1/1098
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Cyclin B1 antibody is available for research use only.
Storage Conditions
Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cytoplasmic & nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cyclin B1 antibody (CCNB1/1098) .

UniProtKB · P14635

G2/mitotic-specific cyclin-B1

CCNB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14635
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCNB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K066, Q5TZP9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell divisionMitosis

Molecular function

Cyclin

Cellular component

CytoplasmCytoskeletonNucleus

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