VCAM-1 Antibody

• Description:

  Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1), L1CAM and INCAM-100) . It is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. VCAM-1 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P19320

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Stimulated human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the VCAM-1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1.4C3

• Applications:

  FACS, IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the VCAM-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This VCAM-1 antibody is available for research use only.

• Storage Conditions:

  Store the VCAM-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membrane

• Image Legend:

  IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with VCAM-1 antibody (clone 1.4C3) .