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Anti-CD14 Antibody

CAT: 0800-V2423-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2423-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P08571
Host
Mouse
Reactivity
Human
Immunogen
Full length human recombinant CD14 protein was used as the antigen for this anti-CD14 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LPSR/553
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the anti-CD14 antibody (with azide) at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This anti-CD14 antibody is available for research use only.
Storage Conditions
Store the anti-CD14 antibody (with azide) at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the anti-CD14 antibody to be titered up or down for optimal performance.
Location
Cell surface, Secreted, Cytoplasmic (Golgi)
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free Anti-CD14 Antibody (clone LPSR/553) . Confirmation of Integrity and Purity of the Antibody.

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