CD14 Antibody

• Description:

  In concert with LBP, CD14 binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

• UniProt:

  P08571

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 292-322 from the human protein was used as the immunogen for this CD14 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, FACS, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Store at 4oC for up to one month. For long term, aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD14 antibody is available for research use only.

• Storage Conditions:

  Store at 4°C for up to one month. For long term, aliquot the CD14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, Secreted, Cytoplasmic (Golgi)

• Image Legend:

  CD14 antibody western blot analysis in A549 lysate. Predicted size 40-55 kDa depending on glycosylation level