CD14 Antibody

• Description:

  Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor) . CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P08571

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment of human CD14 protein (within amino acids 1-200) was used as the immunogen for the CD14 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2 κ

• Clone:

  LPSR/7738

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD14 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD14 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell membrane

• Image Legend:

  IHC staining of FFPE human tonsil tissue with CD14 antibody (clone LPSR/7738) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.