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CD14 Antibody

CAT: 0800-R30515Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30515Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD14, Cluster of differentiation 14, single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P10810
Host
Rabbit
Reactivity
Mouse
Immunogen
An amino acid sequence from the middle region of mouse CD14 (TRDAWLAELQQWLK) was used as the immunogen for this CD14 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the CD14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD14 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cell surface, Secreted, Cytoplasmic (Golgi)
Image Legend
Western blot testing of CD14 antibody and Lane 1: mouse thymus; 2: mouse spleen tissue lysate

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