CD14 Antibody

• Description:

  CD14, Cluster of differentiation 14, single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P10810

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  An amino acid sequence from the middle region of mouse CD14 (TRDAWLAELQQWLK) was used as the immunogen for this CD14 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the CD14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD14 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, Secreted, Cytoplasmic (Golgi)

• Image Legend:

  Western blot testing of CD14 antibody and Lane 1: mouse thymus; 2: mouse spleen tissue lysate