CD11c Antibody

• Description:

  CD11c, also known as Integrin alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute non-lymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Functional studies (order BSA/sodium azide-free format)

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human monocytic U937 phorbol ester-treated cells were used as the immunogen for this CD11c antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  HC1/1

• Applications:

  FACS, IF, Functional studies

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CD11c antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD11c antibody is available for research use only.

• Storage Conditions:

  Store the CD11c antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD11c antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  FACS staining of human PBMCs using CD11c antibody (HC1/1) .