CD117 Antibody

• Description:

  CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. Altered forms of this receptor may be associated with some types of cancer. It is a receptor tyrosine kinase type III, which binds to stem cell factor (a substance that causes certain types of cells to grow), also known as steel factor or c-kit ligand. When this receptor binds to stem cell factor (SCF) it forms a dimer that activates its intrinsic tyrosine kinase activity, that in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. Signalling through CD117 plays a role in cell survival, proliferation, and differentiation. [Wiki]

• Specifications:

  Western blot: 1:1000, Flow Cytometry: 1:10-1:50

• UniProt:

  P10721

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 680-711 from the human protein was used as the immunogen for this CD117 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, FACS, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the CD117 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD117 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD117 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the CD117 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Pig

• CAS Number:

  9007-83-4

• Image Legend:

  CD117 antibody flow cytometric analysis of 293 cells (green) compared to a negative control cell (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.