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Insulin Antibody

CAT: 0800-V2156-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2156-20UGSize:20 µg
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Description
This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. antibody to insulin is an important beta-cell and insulinoma marker.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
Full length purified pig Insulin (aa 1-84) was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
2D11-H5 or INS05
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Insulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Insulin antibody is available for research use only.
Storage Conditions
Store the Insulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC testing of human pancreas stained with Insulin antibody (2D11-H5) . Note cytoplasmic staining of cells.

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