Bcl-2 Antibody

• Description :

  This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl-2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 and the small number in which the neoplastic cells are negative.

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Amino acids GAAPAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  100/D5

• Applications :

  FACS, IF, WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This Bcl-2 antibody is available for research use only.

• Storage Conditions :

  Store the Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, membrane

• Image Legend :

  IHC testing of formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 antibody (100/D5) . Note nuclear membrane & cytoplasmic staining.