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HMGCS1 Antibody

CAT: 0800-RQ6644Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6644Size:100 µg
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Description
Hydroxymethylglutaryl-CoA synthase or HMG-CoA synthase EC 2.3.3.10 is an enzyme which catalyzes the reaction in which acetyl-CoA condenses with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) . Cytoplasmic 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS1) catalyzes the first committed step of mevalonate pathway essential for cholesterol biosynthesis. HMGCS1 transcription is regulated by sterol levels. Studies showed that miR-223 reduces cholesterol biosynthesis by inhibiting HMGCS1 and methylsterol monooxygenase 1. In addition, activation of the EGFR family member ERBB4 induces the expression of SREBP-regulated genes (including HMGCS1) involved in cholesterol biosynthesis, suggesting a role of this enzyme in the metabolic re-programming in ERBB4-driven cancers.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q01581
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids K251-H520) was used as the immunogen for the HMGCS1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, IF, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the HMGCS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HMGCS1 antibody is available for research use only.
Storage Conditions
After reconstitution, the HMGCS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the HMGCS1 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
IHC staining of FFPE human lung cancer tissue with HMGCS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · Q01581

Hydroxymethylglutaryl-CoA synthase, cytoplasmic

HMCS1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q01581
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HMGCS1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HMG-CoA synthase
EC number
2.3.3.10
Processing
—
Secondary accessions
B2RDL8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

Cholesterol biosynthesisCholesterol metabolismLipid biosynthesisLipid metabolismSteroid biosynthesisSteroid metabolismSterol biosynthesisSterol metabolism

Cellular component

Cytoplasm

Disease

Disease variant

Molecular function

Transferase

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