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HMGB1 Antibody

CAT: 0800-RQ5513Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5513Size:100 µg
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Description
High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P09429
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Amino acids DVAKKLGEMWNNTAADDKQPYEKKAAKLKEK were used as the immunogen for the HMGB1 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
5H3
Applications
WB, FACS, IHC-P
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the HMGB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HMGB1 antibody is available for research use only.
Storage Conditions
After reconstitution, the HMGB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the HMGB1 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic, cell membrane, extracellular
Image Legend
IHC staining of FFPE human breast cancer with HMGB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

UniProtKB · P09429

High mobility group protein B1

HMGB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09429
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HMGB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A5D8W9, Q14321, Q5T7C3, Q6IBE1
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationDisulfide bondIsopeptide bondOxidationPhosphoprotein

Biological process

Adaptive immunityAutophagyChemotaxisDNA damageDNA recombinationDNA repairHost-virus interactionImmunityInflammatory responseInnate immunity

Cellular component

Cell membraneChromosomeCytoplasmEndoplasmic reticulumEndosomeMembraneNucleusSecreted

Molecular function

DNA-binding

Domain

Repeat
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