HMGA1 Antibody

• Description:

  HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:1000, Flow Cytometry: 1:10-1:50

• UniProt:

  P17096

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 64-93 from the human protein was used as the immunogen for this HMGA1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IF, WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the HMGA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This HMGA1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the HMGA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the HMGA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat, Hamster

• CAS Number:

  9007-83-4

• Image Legend:

  Fluorescent image of A549 cell stained with HMGA1 antibody at 1:25. HMGA1 immunoreactivity is localized to the nucleus.