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AP2A2 Antibody / AP-2 complex subunit alpha-2

CAT: 0800-RQ6600Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6600Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AP-2 complex subunit alpha-2 is a protein that in humans is encoded by the AP2A2 gene. The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O94973
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids D686-K825) was used as the immunogen for the AP2A2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the AP2A2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This AP2A2 antibody is available for research use only.
Storage Conditions
After reconstitution, the AP2A2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the AP2A2 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear, cell membrane
Image Legend
IHC staining of FFPE human lymphoma tissue with AP2A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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