Products for Research Use Only
RegisterLogin

ORC2 Antibody / Origin recognition complex subunit 2

CAT: 0800-FY12600Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-FY12600Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q13416
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human ORC2 recombinant protein (Position: E130-R493) was used as the immunogen for the ORC2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC, IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This ORC2 antibody is available for research use only.
Storage Conditions
After reconstitution, the ORC2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the ORC2 antibody should be determined by the researcher.
Image Legend
Western blot analysis of ORC2 using anti-ORC2 antibody. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot analysis of multiple cell and tissue lysates probed with anti-ORC2 shows a major band at ~74 kDa, slightly higher than the predicted ~68 kDa, consistent with phosphorylated ORC2. Additional weaker upper and lower bands represent differential phosphorylation states associated with cell-cycle regulation.

Related Products

CatalogName
V7992-100UGORC1 Antibody / Origin Recognition Complex 1
V7992-20UGORC1 Antibody / Origin Recognition Complex 1
RQ7986ORC1 Antibody / Origin Recognition Complex 1
V7992SAF-100UGORC1 Antibody / Origin Recognition Complex 1
RQ8201NCAPH Antibody / Condensin complex subunit 2
RQ6600AP2A2 Antibody / AP-2 complex subunit alpha-2
RQ8929AP2M1 Antibody / AP-2 complex subunit mu
RQ8225NHP2 Antibody / H/ACA ribonucleoprotein complex subunit 2
FY12737PGLYRP2 Antibody / Peptidoglycan recognition protein 2
RQ8607INO80B Antibody / INO80 complex subunit B

Alternative Products

CatalogName
326952-01Origin recognition complex subunit 4
487807ORC2 (Origin Recognition Complex Subunit 2 Ensembl ENSMUSP00000109964, Origin Recognition Complex, Subunit 2)
130738ORC3L (Origin Recognition Complex Subunit 3, Origin Recognition Complex Subunit Latheo, ORC3, LATHEO)
O7050-27CORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6)
130745-APCORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) (APC)
130745-HRPORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) (HRP)
130745-PEORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) (PE)
MBS6148625-01ORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) (FITC)
130744-BiotinORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) (Biotin)
MBS6388075-01ORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) APC

Popular Products