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SRF Antibody / Serum Response Factor

CAT: 0800-RQ6521Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6521Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Serum response factor, also known as SRF, is a transcription factor protein. This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs) . Two transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P11831
Host
Rabbit
Reactivity
Human
Immunogen
An E. coli-derived human protein (amino acids K163-E508) was used as the immunogen for the SRF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the SRF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SRF antibody is available for research use only.
Storage Conditions
After reconstitution, the SRF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SRF antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human endometrial cancer tissue with SRF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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