Serum Response Factor Antibody / SRF

• Description:

  Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.

• Specifications:

  ELISA (order BSA-free format for coating), Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P11831

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the Serum Response Factor antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  PCRP-SRF-1F7

• Applications:

  ELISA, IF, WB, FACS, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Serum Response Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Serum Response Factor antibody is available for research use only.

• Storage Conditions:

  Aliquot the Serum Response Factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Serum Response Factor antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  IHC staining of FFPE human colon tissue with Serum Response Factor antibody (clone PCRP-SF7-1F1) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.