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PARP Antibody / PARP1

CAT: 0800-RQ6271Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6271Size:100 µg
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Description
Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P09874
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant partial protein (amino acids Q670-R858) was used as the immunogen for the PARP antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
2I2H4
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PARP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PARP antibody is available for research use only.
Storage Conditions
After reconstitution, the PARP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PARP antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human poorly differentiated adenocarcinoma of the stomach with PARP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · P09874

Poly [ADP-ribose] polymerase 1

PARP1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09874
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PARP1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PARP-1
EC number
2.4.2.30
Processing
—
Secondary accessions
B1ANJ4, Q8IUZ9
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationIsopeptide bondPhosphoproteinUbl conjugation

Molecular function

Allosteric enzymeDNA-bindingGlycosyltransferaseNucleotidyltransferaseTransferase

Biological process

ApoptosisDNA damageDNA repairImmunityInnate immunityTranscriptionTranscription regulation

Cellular component

ChromosomeCytoplasmNucleus

Ligand

Metal-bindingNADZinc

Domain

RepeatZinc-finger

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