Products for Research Use Only
RegisterLogin

PARP Antibody

CAT: 0800-R31722Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-R31722Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Poly [ADP-ribose] polymerase 1 is composed of four domains: a DNA-binding domain, a caspase-cleaved domain, an auto-modification domain, and a catalytic domain. In vivo, PARP is cleaved by caspases -3 and -7 between aspartic acid 214 and glycine 215, resulting in a 24KD subunit (containing the DNA binding zinc finger motif) and a 89KD subunit (containing the catalytic and auto-modification domains). PARP is an enzyme that modifies nuclear proteins. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human partial recombinant protein (AA 670-858) was used as the immunogen for this PARP antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the PARP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PARP antibody is available for research use only.
Storage Conditions
After reconstitution, the PARP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the PARP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
IHC-P: PARP antibody testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.

Related Products

CatalogName
RQ5291PARP1 Antibody / PARP
F47598-0.4MLPARP 1 Antibody
F48734-0.4MLp-PARP-1 Antibody (pS373)
F40077-0.08MLPARP3 Antibody
RQ6273PARP1 Antibody
R36263-100UGPAR1 Antibody
R34712-100UGPARP2 Antibody
RQ4308PARN Antibody
RQ6109PARP2 Antibody
F47592-0.4MLPARP1 Antibody

Alternative Products

CatalogName
AN200038PPARP-1/PARP Monoclonal Antibody
10R-2170PARP antibody
GWB-94B150PARP, Antibody

Popular Products