Cleaved PARP Antibody

• Description:

  The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. [Wiki] Cleavage of PARP, by enzymes such as caspases or cathepsins, typically inactivates PARP. While in vitro cleavage by caspase occurs throughout the caspase family, preliminary data suggest that caspase-3 and caspase-7 are responsible for in vivo cleavage. Cleavage occurs at aspartic acid 214 and glycine 215, separating PARP into a 24kDA and 89kDA segment. The smaller moiety includes the zinc finger motif requisite in DNA binding. The 89 kDa fragment includes the auto-modification domain and catalytic domain. [Wiki]

• Specifications:

  Western blot: 1:500-1:2000

• UniProt:

  P09874

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human PARP (89kDa cleavage segment) was used as the immunogen for the Cleaved PARP antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  BHH-16

• Type:

  Recombinant

• Applications:

  WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the Cleaved PARP antibody at -20oC.

• Limitations:

  This Cleaved PARP antibody is available for research use only.

• Storage Conditions:

  Store the Cleaved PARP antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the Cleaved PARP antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot testing of human Jukat cell lysate with Cleaved PARP antibody. Predicted molecular weight ~89 kDa (auto-modification/catalytic domain) .