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CYP27A1 Antibody

CAT: 0800-RQ5879Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5879Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CYP27A1 is a gene encoding a cytochrome P450 oxidase, and is commonly known as sterol 27-hydroxylase. It is mapped to 2q35. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
Q02318
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Recombinant human protein (amino acids A7-C531) was used as the immunogen for the CYP27A1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, IHC-P, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the CYP27A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CYP27A1 antibody is available for research use only.
Storage Conditions
After reconstitution, the CYP27A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CYP27A1 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with CYP27A1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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