CYP27B1 Antibody

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CAT#:0800-F54519-0.2MLSize:0.2 mL

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Description

CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

O15528

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A portion of amino acids 482-508 from the human protein was used as the immunogen for the CYP27B1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CYP27B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CYP27B1 antibody is available for research use only.

Storage Conditions

Aliquot the CYP27B1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CYP27B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human kidney tissue with CYP27B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

UniProtKB · O15528

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

CP27B_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: O15528
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CYP27B1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 1.14.15.18
Processing: Precursor
Secondary accessions: B2RC61, Q548T3

Protein keywords

Disease

Disease variant

Ligand

HemeIronMetal-binding

Biological process

Lipid metabolism

Cellular component

MembraneMitochondrion

Molecular function

MonooxygenaseOxidoreductase

Technical term

Proteomics identificationReference proteome

Domain

Transit peptide

Product Datasheet

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