BUB1B Antibody / BubR1

• Description :

  Mitotic checkpoint serine/threonine-protein kinase BUB1 beta is an enzyme that in humans is encoded by the BUB1B gene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt :

  O60566

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant human protein (amino acids K26-E448) was used as the immunogen for the BUB1B antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  5I7

• Applications :

  WB, IHC-P, IF, FACS

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the BUB1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This BUB1B antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the BUB1B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the BUB1B antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  IHC staining of FFPE human breast cancer with BUB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.