BubR1 Antibody / BUB1B

• Description:

  Mitotic checkpoint serine/threonine-protein kinase BUB1 beta is an enzyme that in humans is encoded by the BUB1B gene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  O60566

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant human protein (amino acids K26-E448) was used as the immunogen for the BubR1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  8B3

• Applications:

  WB, IHC-P, FACS

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the BubR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This BubR1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the BubR1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the BubR1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC staining of FFPE human rectal cancer with BubR1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.