BUB1B Antibody

• Description:

  'Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, ' also called BubR1, is an essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. [UniProt]

• Specifications:

  Western blot: 1:1000-1:5000

• UniProt:

  O60566

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human BUB1B was used as the immunogen for the BUB1B antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  ABFG-2

• Type:

  Recombinant

• Applications:

  WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the BUB1B antibody at -20oC.

• Limitations:

  This BUB1B antibody is available for research use only.

• Storage Conditions:

  Store the BUB1B antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the BUB1B antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot testing of human HepG2 lysate with BUB1B antibody. Predicted molecular weight ~120 kDa.