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BubR1 Antibody / BUB1B

CAT: 0800-RQ5566Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5566Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta is an enzyme that in humans is encoded by the BUB1B gene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O60566
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids K26-E448) was used as the immunogen for the BubR1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the BubR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This BubR1 antibody is available for research use only.
Storage Conditions
After reconstitution, the BubR1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the BubR1 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of human 1) HL-60, 2) HEK293, 3) MDA-MB-453, 4) Caco-2 and 5) PC-3 cell lysate with BubR1 antibody. Predicted molecular weight ~120 kDa.
Product Datasheet
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