RAB27A Antibody

• Description :

  Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL

• UniProt :

  P51159

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A human recombinant protein (amino acids L98-K216) was used as the immunogen for the RAB27A antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b

• Clone :

  2F5

• Applications :

  WB, IHC-P, FACS, IF/ICC

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the RAB27A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This RAB27A antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the RAB27A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the RAB27A antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  IHC staining of FFPE human prostate cancer with RAB27A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.