RAB7A Antibody

• Description:

  Ras-related protein Rab-7a is a protein that in humans is encoded by the RAB7A gene. RAB7A functions as a key regulator in endo-lysosomal trafficking, governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positions, and endosome-lysosome transport through different protein-protein interaction cascades. Furthermore, RAB7A is involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes through modulation of SOX10 and the oncogene MYC. Mutations in the lysosomal pathway result in tumor progression in melanoma cells.

• Specifications:

  Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL

• UniProt:

  P51149

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An E.coli-derived human recombinant protein (A156-R197) was used as the immunogen for the RAB7A antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the RAB7A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This RAB7A antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the RAB7A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the RAB7A antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) human U-87 MG, 2) human HEL, 3) rat L6 and 4) mouse C2C12 cell lysate with RAB7A antibody. Predicted molecular weight ~23 kDa.