CD11b Antibody / MAC-1 / ITGAM

• Description:

  The ITGAM gene encodes the integrin alpha M chain, also called CD11b and MAC-1. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3') . The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [RefSeq]

• Specifications:

  Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200

• UniProt:

  P11215

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A synthetic peptide specific to human CD11b / ITGAM was used as the immunogen for the CD11b antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  GC-9

• Type:

  Recombinant

• Applications:

  IHC-P, WB

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the CD11b antibody at -20oC.

• Limitations:

  This CD11b antibody is available for research use only.

• Storage Conditions:

  Store the CD11b antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the CD11b antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Image Legend:

  IHC staining of FFPE human spleen tissue with CD11b antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.