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NOX5 Antibody

CAT: 0800-RQ4593Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4593Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NOX5 (Nadph Oxidase 5) is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)
UniProt
Q96PH1
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids E13-F197 from the human protein were used as the immunogen for the NOX5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF/ICC, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the NOX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Storage Conditions
After reconstitution, the NOX5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NOX5 antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) HeLa, 2) placenta, 3) SCG-7901, 4) Jurkat and 5) THP-1 lysate with NOX5 antibody at 0.5ug/ml. Predicted molecular weight: ~86 kDa.

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