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NOX1 Antibody

CAT: 0800-R30751Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30751Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NADPH OXIDASE 1, also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. It is expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of NOX1 in NIH3T3 cells increased superoxide generation and cell growth. Cells expressing NOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
Q9WV87
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
An amino acid sequence from the middle region of rat NOX1 (WYKFQRAHNKLKTQK) was used as the immunogen for this NOX1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the NOX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NOX1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NOX1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the NOX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC staining of FFPE rat colon tissue with NOX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · Q9WV87

NADPH oxidase 1

NOX1_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q9WV87
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Nox1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
NOX-1
EC number
1.6.3.-
Processing
—
Secondary accessions
—
Protein keywords

Cellular component

Cell junctionCell membraneCell projectionMembrane

Biological process

Electron transportTransport

Ligand

FADFlavoproteinHemeIronMetal-bindingNADP

PTM

GlycoproteinPhosphoprotein

Molecular function

Oxidoreductase

Technical term

Reference proteome

Domain

TransmembraneTransmembrane helix

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