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ADAM10 Antibody

CAT: 0800-RQ4035Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4035Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ADAM10, A Disintegrin and Metalloproteinase Domain 10, is also known as AD10. ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function. The ADAM10 gene is mapped to chromosome 15q21.3-q23. ADAM proteins contain an N-terminal signal sequence, followed by a prodomain, a metalloprotease-like domain, a disintegrin-like domain, a cysteine-rich region, an EGF -like repeat, a transmembrane domain, and a C-terminal cytoplasmic tail. Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase. ADAM10 possesses TNFA convertase activity.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O14672
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human partial protein corresponding to amino acids T214-D325 was used as the immunogen for the ADAM10 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the ADAM10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ADAM10 antibody is available for research use only.
Storage Conditions
After reconstitution, the ADAM10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ADAM10 antibody should be determined by the researcher.
Image Legend
Western blot testing of rat 1) thymus and 2) stomach tissue lysate with ADAM10 antibody at 0.5ug/ml. Predicted molecular weight: ~84 kDa (full length), ~60 kDa (active form), ~80 kDa (glycosylated active form), ~110 kDa (glycosylated full length) .
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