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MED18 Antibody

CAT: 0800-R32989Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32989Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 directly interacted with the Mediator subunit TRFP (MED20) . The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)
UniProt
Q9BUE0
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids M1-M208 was used as the immunogen for the MED18 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the MED18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MED18 antibody is available for research use only.
Storage Conditions
After reconstitution, the MED18 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MED18 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of human 1) A431, 2) K562, 3) U-2 OS, 4) HL60, 5) rat liver and 6) mouse kidney lysate with MED18 antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.

UniProtKB · Q9BUE0

Mediator of RNA polymerase II transcription subunit 18

MED18_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BUE0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MED18
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
D3DPM1, Q9NXU9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Activator

Cellular component

Nucleus

PTM

Phosphoprotein

Biological process

TranscriptionTranscription regulation
Product Datasheet
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