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MED8 Antibody

CAT: 0800-R32979Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32979Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene. This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA (recombinant human protein) : 0.1-0.5 µg/mL (BSA-free format available)
UniProt
Q96G25
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids M1-R195 was used as the immunogen for the MED8 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, Direct ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the MED8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MED8 antibody is available for research use only.
Storage Conditions
After reconstitution, the MED8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MED8 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of 1) rat pancreas, 2) mouse pancreas and 3) human U-2 OS lysate with MED8 antibody at 0.5ug/ml. Expected molecular weight: 29-32 kDa.

UniProtKB · Q96G25

Mediator of RNA polymerase II transcription subunit 8

MED8_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96G25
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MED8
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A9IZ91, A9IZ92, Q5JUY8, Q96FQ4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

Activator

Coding sequence diversity

Alternative splicing

Domain

Coiled coil

Cellular component

Nucleus

PTM

Phosphoprotein

Biological process

TranscriptionTranscription regulationUbl conjugation pathway

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