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Med12 Antibody

CAT: 0800-F54762-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54762-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity) .
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25
UniProt
A2AGH6
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1991-2025 from the mouse protein was used as the immunogen for the Med12 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Med12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Med12 antibody is available for research use only.
Storage Conditions
Aliquot the Med12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Med12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Western blot testing of mouse NIH 3T3 cell lysate with Med12 antibody. Predicted molecular weight ~245 kDa.
Product Datasheet
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