Trem1 Antibody

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by TREM1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF) ; MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40) . Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

9007-83-4

Western Blot: 0.5-1 µg/mL, ELISA (Capture; recombinant mouse protein) : 0.1-0.5 µg/mL (BSA-free format available)

Q9JKE2

Mouse, Rat

A recombinant mouse protein corresponding to amino acids A21-S202 was used as the immunogen for the Trem1 antibody.

WB, ELISA (capture)

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

After reconstitution, the Trem1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

This Trem1 antibody is available for research use only.

After reconstitution, the Trem1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Trem1 antibody should be determined by the researcher.