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CDC25C Antibody

CAT: 0800-R32056Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R32056Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P30307
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids 175-473 of human CDC25C were used as the immunogen for the CDC25C antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CDC25C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CDC25C antibody is available for research use only.
Storage Conditions
After reconstitution, the CDC25C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CDC25C antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with CDC25 antibody (green) and Alpha Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.

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