Cdc25A Antibody

• Description:

  Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. [UniProt]

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

• UniProt:

  P30304

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 271-300 from the human protein was used as the immunogen for this Cdc25A antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Store at 4oC for up to one month. For long term, aliquot the Cdc25A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Cdc25A antibody is available for research use only.

• Storage Conditions:

  Store at 4°C for up to one month. For long term, aliquot the Cdc25A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Cdc25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC analysis of FFPE human spleen tissue stained with Cdc25A antibody