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PP2A Antibody (catalytic subunit) / PPP2CA

CAT: 0800-R31760Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31760Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human recombinant PPP2CA protein (AA 1-309) was used as the immunogen for this PP2A antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PP2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PP2A antibody is available for research use only.
Storage Conditions
After reconstitution, the PP2A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the PP2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with PP2A antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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