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PPP2CA Antibody / PP2A alpha

CAT: 0800-RQ5853Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5853Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P67775
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Recombinant human protein (amino acids M1-L309) was used as the immunogen for the PPP2CA antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
3B6
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PPP2CA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PPP2CA antibody is available for research use only.
Storage Conditions
After reconstitution, the PPP2CA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PPP2CA antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human HeLa cells with PPP2CA antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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