PPP2CA Antibody / PP2A alpha

• Description:

  The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  P67775

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Monkey

• Immunogen:

  Recombinant human protein (amino acids M1-L309) was used as the immunogen for the PPP2CA antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  3B6

• Applications:

  WB, IHC-P, IF, FACS

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the PPP2CA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This PPP2CA antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PPP2CA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the PPP2CA antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of FFPE human HeLa cells with PPP2CA antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.