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PP2Ac Antibody (catalytic subunit) / PPP2CA

CAT: 0800-R30249Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30249Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of PPP2CA is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL
UniProt
P67775
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of human PPP2CA (FTKELDQWIEQLNEC) was used as the immunogen for this PP2Ac antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
The lyophilized PP2Ac antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.
Limitations
This PP2Ac antibody is available for research use only.
Storage Conditions
The lyophilized PP2Ac antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Variations in secondary/substrate sensitivities and test protocols may require the PP2Ac antibody to be titrated for optimal performance.
Image Legend
IHC staining of FFPE human breast cancer with PP2Ac antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.

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